FastQCFastQC Report
Fri 16 Jan 2026
M6D17_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM6D17_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5616
Sequences flagged as poor quality0
Sequence length247-251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA66111.769943019943021No Hit
CCTGTTCGATACCCACACTTTCGTGCATGAGCGTCAGTTGAGCGCCGGTA5589.935897435897436No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGCGCGCCGGTA4698.3511396011396No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA3866.873219373219373No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG2975.288461538461538No Hit
CCTGTTCGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGAA2935.217236467236467No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTAGGGCGCCGGTA2865.092592592592593No Hit
CCTGTTCGATCCCCACGCTTTCGTGCTTCAGCGTCAGTAGGAAGCCGGCA2494.433760683760684No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA1923.418803418803419No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG1913.400997150997151No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA1312.3326210826210825No Hit
CCTGTTTGCTCCCCATGCTCTCATGCCTCAGCGTCAGTTACAGTCCAGTA1152.047720797720798No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGTCGTAGTTTGGTA771.371082621082621No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGGGCGCCGGTA741.3176638176638178No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGGGCGCCGGTA691.2286324786324787No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAATGTCAGTATCGACCCAGCA520.9259259259259258No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGCA430.7656695156695157No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCTGGCGCCGGTA400.7122507122507122No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA380.6766381766381766No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTCGGGCGCCGGTA360.641025641025641No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGATAGGAGCCGGTA290.5163817663817664No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA270.4807692307692308No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGACAGGAGCCGGTA220.39173789173789175No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGTGTCAGTTACAGTCCAGAA200.3561253561253561No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA200.3561253561253561No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACGGTCCAGTA190.3383190883190883No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA190.3383190883190883No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTTGCAGTCCAGTT170.30270655270655267No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTG150.2670940170940171No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCACCGTCCAGCA150.2670940170940171No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTTCTGTCCAGCA140.24928774928774927No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTC140.24928774928774927No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTGACGGCCCAGAG130.23148148148148145No Hit
CCTGTTTGCTCCCCACGCTCTCGAGCCTCAACGTCAGTCACGGTCCAGCA130.23148148148148145No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA130.23148148148148145No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA130.23148148148148145No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAGTCGTCCAGTA130.23148148148148145No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA120.2136752136752137No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA110.19586894586894588No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCGCCGTCCAGCA110.19586894586894588No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT100.17806267806267806No Hit
CCTGTTTGCTACCCACGCTTTCGTGCTTCAGCGTCAGTTAAAGCCCAGCA90.16025641025641024No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCA90.16025641025641024No Hit
CCTGTTTGCTCCCCACGCTTTCGGGCCTCAACGTCAGTCGCAGTCCAGCA80.14245014245014245No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTTCAGTCCAGCA80.14245014245014245No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGGGTCAGTTGCAGTCCAGTG80.14245014245014245No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA70.12464387464387464No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA70.12464387464387464No Hit
CCTGTTCGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG70.12464387464387464No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTAGGAAGCCGGCA70.12464387464387464No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTATCGTCCAGTA70.12464387464387464No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA70.12464387464387464No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCAGTCCAGTA70.12464387464387464No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA70.12464387464387464No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGAGCGCCGGTA60.10683760683760685No Hit
CCTGTTCGATACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG60.10683760683760685No Hit
CCTGTTCGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA60.10683760683760685No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGTA60.10683760683760685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)