FastQCFastQC Report
Fri 16 Jan 2026
M4D5_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM4D5_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4749
Sequences flagged as poor quality0
Sequence length247-251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGTCGTAGTTTGGTA69614.655716993051168No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA3637.643714466203411No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA2896.085491682459465No Hit
CCTGTTCGATACCCACACTTTCGTGCATGAGCGTCAGTTGAGCGCCGGTA1954.10612760581175No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGCGCGCCGGTA1843.8744998947146767No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA1553.2638450200042115No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTTGCAGTCCAGTT1523.200673826068646No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTAGGGCGCCGGTA1433.0111602442619496No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA1332.800589597810065No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG1252.6321330806485577No Hit
CCTGTTCGATCCCCACGCTTTCGTGCTTCAGCGTCAGTAGGAAGCCGGCA1132.379448304906296No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA992.0846493998736575No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA962.021478205938092No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA771.6213939776795114No Hit
CCTGTTTGCTCCCCACGCTTTCGGGCCTCAACGTCAGTCGCAGTCCAGCA721.5161086544535691No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACGGTCCAGTA651.36870920193725No Hit
CCTGTTCGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGAA621.3055380080016845No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCGCCGTCCAGCA501.052853232259423No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACTGTCCAGTA491.0317961676142346No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGCA481.010739102969046No Hit
CCTGTTTGCTCCCCATGCTCTCATGCCTCAGCGTCAGTTACAGTCCAGTA470.9896820383238577No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGTCGTCCAGCA430.9054537797431037No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTTCAGTCCAGCA430.9054537797431037No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA430.9054537797431037No Hit
CCTGTTTGCTACCCACGCTTTCGTGCTTCAGCGTCAGTTAAAGCCCAGCA430.9054537797431037No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCA430.9054537797431037No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA390.82122552116235No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGCA380.8001684565171615No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA370.779111391871973No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA350.7369972625815961No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGGGCGCCGGTA320.6738260686460307No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTC310.6527690040008423No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA300.6317119393556538No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTATCGTCCAGTA270.5685407454200885No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTG260.5474836807749No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA240.505369551484523No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTACACCGTCCAGC220.4632554221941461No Hit
CCCGTTCGCTACCCACGCTTTCGCGCCTCAGCGTCAGGTACAGTCCAGAA220.4632554221941461No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCACCGTCCAGCA210.4421983575489577No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA200.4211412929037693No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA200.4211412929037693No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTG180.3790271636133923No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA180.3790271636133923No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTTACAGTCCAGTA160.33691303432301534No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTTCTGTCCAGCA150.3158559696778269No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCTGGCGCCGGTA150.3158559696778269No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCAGTCCAGTA140.29479890503263845No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG130.27374184038745No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA120.2526847757422615No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA120.2526847757422615No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGCAGTCCAGCA110.23162771109707306No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA100.21057064645188464No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACGGTCCAGCA100.21057064645188464No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTGACGGCCCAGAG100.21057064645188464No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA100.21057064645188464No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCGCGGTCCAGCA100.21057064645188464No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTTGGCCCAGCA90.18951358180669614No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCTAGCA90.18951358180669614No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAGAGCCCAGCA90.18951358180669614No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAGTTTCAGTCCAGAA80.16845651716150767No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAGTCGTCCAGTA80.16845651716150767No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTCGGGCGCCGGTA70.14739945251631922No Hit
CCTGTTTGCTCCCCACGCCTTCGGGCCTCAACGTCAGTCACAGTCCAGCA70.14739945251631922No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGTGTCAGTTACAGTCCAGAA60.12634238787113075No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCATCAACGTCAGTCGCCGTCCAGTA60.12634238787113075No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGGGCGCCGGTA60.12634238787113075No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTG60.12634238787113075No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTTGCAGTCCAGTT60.12634238787113075No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA50.10528532322594232No Hit
CCTGTTTGCTCCCCACGCTCTCGAGCCTCAACGTCAGTCACGGTCCAGCA50.10528532322594232No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGTCGCAGTTTGGTA50.10528532322594232No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)