FastQCFastQC Report
Fri 16 Jan 2026
M3D3_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM3D3_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875
Sequences flagged as poor quality0
Sequence length250-251
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA24528.000000000000004No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA16318.628571428571426No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA14716.8No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA728.228571428571428No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTA374.228571428571429No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA313.5428571428571427No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCACTGGGCGTAAAGGGAGCGCA192.1714285714285713No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA182.057142857142857No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGCGCA141.6No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCA121.3714285714285714No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA70.8No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA70.8No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA70.8No Hit
TACGTAGGGAGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGCGTA70.8No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA50.5714285714285714No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA50.5714285714285714No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCA40.4571428571428572No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA40.4571428571428572No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATCACTGGGTGTAAAGGGAGCGTA40.4571428571428572No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCACTGGGTGTAAAGGGAGCGTA30.34285714285714286No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA20.2285714285714286No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGAGAGCGTA20.2285714285714286No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA20.2285714285714286No Hit
TACGTAGGGAGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA20.2285714285714286No Hit
NACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA20.2285714285714286No Hit
TACGTAGGTGGCAAGCGTTGTTCGGAATAACTGGGCGTAAAGGATGCGTA20.2285714285714286No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA20.2285714285714286No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCACTGGGCGTAAAGGGAGCGCA20.2285714285714286No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA20.2285714285714286No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA20.2285714285714286No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGCGTGTA20.2285714285714286No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGTACGTA20.2285714285714286No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA20.2285714285714286No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGAATAGGGAGCGTA10.1142857142857143No Hit
TACGGAGGATGCGAGCGTTGTCCGGATTTATTGGGCTTAAAGGGAGCGCA10.1142857142857143No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA10.1142857142857143No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAACGCGCGCTTC10.1142857142857143No Hit
GACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA10.1142857142857143No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGCGTA10.1142857142857143No Hit
TACGTAGGAGGCAAGCGTTATCCGGAATGACTGGGCGTAAAGGGTGCGTA10.1142857142857143No Hit
NACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA10.1142857142857143No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGCGTA10.1142857142857143No Hit
TACGTAGGGGGCAAACGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA10.1142857142857143No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGGGTAAAGGGAGCGTA10.1142857142857143No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGCA10.1142857142857143No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA10.1142857142857143No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGAGCGTA10.1142857142857143No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTGAAGGGTGCGCA10.1142857142857143No Hit
TACGGAGGATGCGAGCGTTATCCGGATTGATTGGGTTTAAAGGGGGCGTA10.1142857142857143No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGATTTAAAGGGTGCGTA10.1142857142857143No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA10.1142857142857143No Hit
CACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA10.1142857142857143No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCACTAGGCGTAAAGGGAGCGCA10.1142857142857143No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGGGCGTA10.1142857142857143No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGCGTA10.1142857142857143No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCA10.1142857142857143No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA10.1142857142857143No Hit
NACGGAGGATGCGAGCGTTATCCGGATTTATTTGGTTTAANGNGNGNNNA10.1142857142857143No Hit
TACGGAGGATGCGAGCATTATCCGGATTTATTGGGTTTAAAGGGTGCGTA10.1142857142857143No Hit
NACGTAGGGGGCAAGCGTTATCCAGATTTACTGGGTGTAAAGGGAGCGTA10.1142857142857143No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATGACTGGGCGTAAAGGGAGTGTA10.1142857142857143No Hit
GACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTGAAAGGGTGCGCA10.1142857142857143No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTCTAAAGGGTGCGCA10.1142857142857143No Hit
TACGTAGGGAGCAAGCGTTGTTCGGAATGACTGGGCGTAAAGGGTGCGTA10.1142857142857143No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGGAAAGGGAGCGTA10.1142857142857143No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTAGGTGTAAAGGGAGCGTA10.1142857142857143No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTACAGGGAGCGTA10.1142857142857143No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGAGTA10.1142857142857143No Hit
TACGGAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA10.1142857142857143No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTA10.1142857142857143No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA10.1142857142857143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)