FastQCFastQC Report
Fri 16 Jan 2026
M3D149_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM3D149_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length248-251
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATCCCCACACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGAA214.285714285714285No Hit
CCTGTTCGATACCCACACTTTCGTGCATGAGCGTCAGTTGAGCGCCGGTA17.142857142857142No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAGTTGCCCAGAG17.142857142857142No Hit
CCTGTTCGATCCCCACGCTTTCGTCCTTCAGCGTCAGTCGGGCCCCGGTA17.142857142857142No Hit
CCTGTTTGCTCCCCGCGCTTTCGAACCTCAGCGTCAATACACCACCAGTT17.142857142857142No Hit
CCTGTTCGATACCCGCGCTTTAGTGCCTCAGCGTCAGTCGGGCGCCGGTC17.142857142857142No Hit
CCAGGCCGCTCCACAAGATTTCGATCATAGGTGTCCGTGCCGGCTCAGCC17.142857142857142No Hit
CCTGTTCGATACCCACACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGAA17.142857142857142No Hit
CCTGTTCGATACCCACACTTTCGTGCTTCAGCGTCAGTAGGGCGCCGGTA17.142857142857142No Hit
CCCATTTGATCCCCTAGCTTTCGTGCCTCAGTGTCAGTTATGGTCCAGCA17.142857142857142No Hit
CCTGTTCGCTACCCACGCTTTCGCTCCTCAGTGTCAGTAATGGCCCAGAG17.142857142857142No Hit
CCCGTTTGCTACCTTGGCTTTCGTGCCTCAGTGTCAGGAATTGTCCAGAG17.142857142857142No Hit
ACCCAACAAAACAGACTCCAACGAGGCTACTAAAAAGCCTGACGCCGTCC17.142857142857142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)