FastQCFastQC Report
Fri 16 Jan 2026
M3D149_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM3D149_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA535.714285714285715No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA214.285714285714285No Hit
TACGTAGGGTGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA17.142857142857142No Hit
TACAGAGGTCCCGAGCGTTGTTCGGATTCACTGGGCGTAAAGGGTGCGTA17.142857142857142No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTCTGTA17.142857142857142No Hit
TACATAGGGTGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA17.142857142857142No Hit
TACATAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA17.142857142857142No Hit
TACGAAAGGTGCAAGCGTTGTTCGGATTGACTGGGCGTAAAGAGCACGTA17.142857142857142No Hit
TACAGAGGATGCAAGCGTTATCCGGAATCACTGGGCATAAAGCGTCTGTA17.142857142857142No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)