FastQCFastQC Report
Fri 16 Jan 2026
M2D19_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM2D19_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length249-251
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA45.633802816901409No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGCGCGCCGGTA45.633802816901409No Hit
CCTGTTCGATACCCACACTTTCGTGCATGAGCGTCAGTTGAGCGCCGGTA34.225352112676056No Hit
CCTGTTCGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGAA34.225352112676056No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA22.8169014084507045No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA22.8169014084507045No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTTGGGCGCCGGTA22.8169014084507045No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTCGGGCGCCGGTA22.8169014084507045No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCTGGCGCCGGTA22.8169014084507045No Hit
CCTGTTCGATCCCCGCACTTTCGTGCATGAGCGTCAGTTGAGCGCCGGTA11.4084507042253522No Hit
CCTGTTCGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA11.4084507042253522No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGTGTCAGTTGCAGGCCAGTA11.4084507042253522No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA11.4084507042253522No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTTGCACGCCGGCA11.4084507042253522No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAAGGCGCCGGTA11.4084507042253522No Hit
TCAGATTAATACGCACGCTTCTGTGGGTAAGTATAACTGGTGCCTCGGGA11.4084507042253522No Hit
ACTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGCGCGCCGGTA11.4084507042253522No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTCGGGGGTTGGTA11.4084507042253522No Hit
CCTGTTCGATCCCCGCACTTTCGTGCTTCAGCGTCAGTAGGGCGCCGGAA11.4084507042253522No Hit
CCTGTTTGATCCCCACACTTTCGAGCCTCAGCGTCAGTAACGGTCCGGTA11.4084507042253522No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGTGTCAGTTGCAGTCCGGTG11.4084507042253522No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCNGCA11.4084507042253522No Hit
CCTGTTCGATACCCACACTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA11.4084507042253522No Hit
CCTATTCGAGACCCACGCTTTCGTGCATCAGATTCAGTTGAACGCAGGCA11.4084507042253522No Hit
CCTGTTTGCTCCCCATGCTCTCATGCCTCAGCGTCAGTTACAGTCCAGTA11.4084507042253522No Hit
TCTGTTTGCTCACAACGCTCGCGAGGATCAACATCCTTTTCTGTCCCTCA11.4084507042253522No Hit
CCTGTTCGATACCCACACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGAA11.4084507042253522No Hit
CTTGTTAGATCCCCACGCTTTCGAGCCGCTCCGAAAATACGTTTCAAGTA11.4084507042253522No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTTGCAGTCCAGCA11.4084507042253522No Hit
CCTGTTTGATCCCCCCGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA11.4084507042253522No Hit
CCTGTTCGATACCCACGCTTTCGTGCATCAGCGTCAGTAGGGCGCCGGTA11.4084507042253522No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTTGCGCGCCGGTA11.4084507042253522No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTGACGGCCCAGAA11.4084507042253522No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTACAGCCCAGTA11.4084507042253522No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA11.4084507042253522No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAC11.4084507042253522No Hit
CCTGTTCGCTACCCACGCTGTCGTGAAGGAGCGTCAGGTGGGGTCGGGTA11.4084507042253522No Hit
CCTGTTAGATCCCCCCCCTTTCGTGCTTCAGCGTCAGTAGGGCGCCGGTA11.4084507042253522No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG11.4084507042253522No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTC11.4084507042253522No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGTCGTAGGTTGGTA11.4084507042253522No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCATCAGCGTCAGTTTAGCCCCAGAG11.4084507042253522No Hit
CCTGTTCGATACCCACGCTTTCGAGCATCAGCGTCAGTTGCAGTCCGGTA11.4084507042253522No Hit
CCTGTTCGATACCCACGCTTTCGTGCATCAGCGTCAGTAGCGCGCCGGTA11.4084507042253522No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTAAACCCCAGCA11.4084507042253522No Hit
CCTGTTCGATACCCACACTTTCGTGCATCAGCGTCAGTCGGGCGCCGGTA11.4084507042253522No Hit
CCTGTTCGATACCCACACTTTCGTGCCTCAGCGTCAGTAGCGCGCCGGTA11.4084507042253522No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTAGGGCGCCGGTA11.4084507042253522No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTCGCGGGCTGGTA11.4084507042253522No Hit
CCTCTTTGCTCCCCCCGCTTTCGACCCTCAACGTCAGTTACAGTCCAGTG11.4084507042253522No Hit
CCTGTTCGATACCCACACTTTCGTGCATGAGCGTCAGTTGCGCGCCGGTA11.4084507042253522No Hit
CCTGTTCGATACCCACACTTTCGTGCATGAGCGTCAGTTGGACGCCGGTA11.4084507042253522No Hit
CCTGTTCGATCCCCACGCTTTCGTGCTTCAGCGTCAGTCGGGCGCCGGTA11.4084507042253522No Hit
CATGTTCGATACCCGCACTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGAA11.4084507042253522No Hit
CCTGTTTGATCCACACTCTTTCGTGCTTCAACGTCAGTCGTAGTTTGGCA11.4084507042253522No Hit
CCTGTTCGATCCCCACGCTTACGTGCTTCAGCGTCAAAAGGGAGCCGGCA11.4084507042253522No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)