FastQCFastQC Report
Fri 16 Jan 2026
M2D125_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM2D125_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485
Sequences flagged as poor quality0
Sequence length250-251
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA19540.20618556701031No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA12024.742268041237114No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA244.948453608247423No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA193.917525773195876No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA122.4742268041237114No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA102.0618556701030926No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGCGCA102.0618556701030926No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTA71.443298969072165No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA40.8247422680412372No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA40.8247422680412372No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA40.8247422680412372No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCA30.6185567010309279No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA30.6185567010309279No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCACTGGGCGTAAAGGGAGCGCA20.4123711340206186No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA20.4123711340206186No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA20.4123711340206186No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA20.4123711340206186No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA20.4123711340206186No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA20.4123711340206186No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA20.4123711340206186No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCGCGCA20.4123711340206186No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA20.4123711340206186No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTATTGGGTGTAAAGCGAGCGCA10.2061855670103093No Hit
TTAGAAACCCTTGTAGTCCGGCTGACTGACTTATAGCGAATATCGTATGC10.2061855670103093No Hit
TACGTAGGATCCGAGCATTATCCGGAGTGACTGGGTGTAAAGAGTTGCGT10.2061855670103093No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGTA10.2061855670103093No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCA10.2061855670103093No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGCTTAAAGGGAGCGTA10.2061855670103093No Hit
NACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAANNNNNNNNNN10.2061855670103093No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTACTGGGCGTAAAGAGCGCGCA10.2061855670103093No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGAGCGCGCA10.2061855670103093No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGAGCGTA10.2061855670103093No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGGGAGCGCA10.2061855670103093No Hit
TACGTAAGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGAGTA10.2061855670103093No Hit
TACGTAGGAGGCAAGCGTTATCCGGAATGACTGGGCGTAAAGGGTGCGTA10.2061855670103093No Hit
TACGGAGGATTCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA10.2061855670103093No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTGAAAGGGTGCGTA10.2061855670103093No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATGACTGGGCGTAAAGGGTGCGTA10.2061855670103093No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGGGTAAAGGGAGCGTA10.2061855670103093No Hit
TACGTAGGGGGCAAGCGTTGTTCGGATTTACTGGGTGTAAAGGGTGCGTA10.2061855670103093No Hit
TACGTAGGTGGCAAGCGTTGTTCGGAATAACTGGGCGTAAAGGATGCGTA10.2061855670103093No Hit
TACGGAGGGTGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA10.2061855670103093No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCACTGGGTGTAAAGGGAGCGTA10.2061855670103093No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA10.2061855670103093No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGAGCGTA10.2061855670103093No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGCA10.2061855670103093No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTAACTGGGCGTAAAGGGCGTGTA10.2061855670103093No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGGGCGCA10.2061855670103093No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGCTTAAAGGGCGCGTA10.2061855670103093No Hit
TACGTAGGGGTCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA10.2061855670103093No Hit
TACGGAGGATTCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA10.2061855670103093No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGCGCGTA10.2061855670103093No Hit
TACGGAGGAGGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA10.2061855670103093No Hit
TACGTAGGTGTCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCA10.2061855670103093No Hit
TACGGAGGATCCGAGCATTATCCGGATTGACTGGGTGTAAAGAGTTGCGA10.2061855670103093No Hit
TACGTAGGAGGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGAGCGTA10.2061855670103093No Hit
TACGGAGGGTGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA10.2061855670103093No Hit
TACGTAGGGTGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGCTCGTA10.2061855670103093No Hit
TACATAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA10.2061855670103093No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTACTGGGTTTAAAGGGAGCGTA10.2061855670103093No Hit
TACGGAGGATGCGAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTA10.2061855670103093No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA10.2061855670103093No Hit
TACGGAGGATGCGAGCATTATCCGGATTTATTGGGTTTAAAGGGTGCGTA10.2061855670103093No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA10.2061855670103093No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGCA10.2061855670103093No Hit
TACGGAGGATGCGAGCGTTATCTGGATTTATTGGGTTTAAAGGGTGCGCA10.2061855670103093No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTACTGGGTTTAAAGGGAGCGTA10.2061855670103093No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTACTGGGTTTAAAGGGTGCGTA10.2061855670103093No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA10.2061855670103093No Hit
TACGGAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA10.2061855670103093No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA10.2061855670103093No Hit
TACGTAGGAGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA10.2061855670103093No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTTTAAAGGGAGCGTA10.2061855670103093No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA10.2061855670103093No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)