FastQCFastQC Report
Fri 16 Jan 2026
M1D9_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM1D9_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70
Sequences flagged as poor quality0
Sequence length249-251
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA57.142857142857142No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA34.285714285714286No Hit
CCTGTTCGATACCCACACTTTCGTGCATGAGCGTCAGTTGAGCGCCGGTA34.285714285714286No Hit
CCTGTTCGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGAA34.285714285714286No Hit
CCTGTTCGATCCCCACGCTTTCGTGCTTCAGCGTCAGTAGGAAGCCGGCA22.857142857142857No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGCG22.857142857142857No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG22.857142857142857No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTAGGGCGCCGGTA22.857142857142857No Hit
CCTGTTCGCTCCCCACGCTTTCGGGCCTCAACGTCAGTTGCCGTCCAGTA11.4285714285714286No Hit
TCTGTTAGATACCCAAACTTTAGACCAAAAGAGTAAAATGCAGTCCCGTA11.4285714285714286No Hit
CCTGTTCGATCCCCACGCTTTCGTGCTTCAGCGTCAGTCTGGCGCCGGTA11.4285714285714286No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCATCAGTAGTAGTCCGGTA11.4285714285714286No Hit
CCTGTTCGATCCCCACGCTTTCGTGCTTCAGCGTCAGTTGCAAGCCGGCA11.4285714285714286No Hit
CCTGTTAGCTCCACACGCTTACGTGAGTCAAAGTCAGTCGTAGTTTGGTA11.4285714285714286No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCATCAGCGTCAGTTACCGCCCAGCA11.4285714285714286No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA11.4285714285714286No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA11.4285714285714286No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAACGTCAGTCGTACTTTGGTA11.4285714285714286No Hit
CCTGTCTGATCACCGCCCTTACAAGCCTCAGCGTCAGTAACGCGCCGGTA11.4285714285714286No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTAACAGGCCAGAG11.4285714285714286No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGAGCCGGTA11.4285714285714286No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA11.4285714285714286No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA11.4285714285714286No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCGCCGTCCAGCA11.4285714285714286No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTGCAGTCCAGTG11.4285714285714286No Hit
AATGTTCGATACACACGCCTTCGAGCCTCAGCGTCACTATTGATCCTGTA11.4285714285714286No Hit
TCTGTTTTCTCCCCACGCTCTCTTACATCAGCGTCAATTTTTGCCCGGAT11.4285714285714286No Hit
CATGTTTGATCCAGACGCTTGAGAGTCTCATTGTCAGTTACTGTCCAGCA11.4285714285714286No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTTGGGCGCCGGTA11.4285714285714286No Hit
CCTGTTCGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAGCGGGCCCGAA11.4285714285714286No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTCGGAGGTCGGTA11.4285714285714286No Hit
CCTGTTCGATACCCACACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA11.4285714285714286No Hit
CCTGTTTGCTCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGAA11.4285714285714286No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCTGGCGCCGGTA11.4285714285714286No Hit
CCTGTTCGCTACCCATGCTTTCGACCCTCAGCGTCAGTGATGGCCCAGCC11.4285714285714286No Hit
CCTGTACGATACCCACGCTTTCGTGCATGAGCGTCAGTTGCGCGCCGGTA11.4285714285714286No Hit
CATGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGCA11.4285714285714286No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCA11.4285714285714286No Hit
CCTGTTCGATCCCCACGCTTTCGTGCTTCAGCGTCAGTAGCACGCCGGTA11.4285714285714286No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCATCAACGTCAGTTACGGGCCGGTA11.4285714285714286No Hit
CCTGTTTGCTCNCCATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11.4285714285714286No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA11.4285714285714286No Hit
CCTGTTCGATACCCACGCTTTCGTGCATCAGCGTCAGTTGCACGCCAGTA11.4285714285714286No Hit
CCTGTTCGATACCCACGCTTTCGCGCCTCAGCGTCAGTTACGCGCCCGCA11.4285714285714286No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTGAGCGTCAGTTGGGCGCCGGTA11.4285714285714286No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTCGCCCTCCGGTA11.4285714285714286No Hit
CCTGTTTGATCCCCAGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGTA11.4285714285714286No Hit
CCTATTTGATCCCCACGCTTACGTACCTCAGGGTCAGTTACGGTCCGGTA11.4285714285714286No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGCGCGCCGGTA11.4285714285714286No Hit
CCTGTTTGCTCCCCATGCTCTCTTGCCTCAGCGTCAGTTCCAGTCCAGTA11.4285714285714286No Hit
CCTGTTTGATACCCACACTTTCGTGCATCAGTGTCAGTAGGGGGCCGGTA11.4285714285714286No Hit
CCTGTTCGATACCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGAA11.4285714285714286No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTTAGCCCAGCA11.4285714285714286No Hit
CCTGTTCGATACCCACGNNTNNGTNNNNNANNNNCAGTNNAGCGNNNNNN11.4285714285714286No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA11.4285714285714286No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA11.4285714285714286No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)