FastQCFastQC Report
Fri 16 Jan 2026
M1D149_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM1D149_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length248-251
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA67.228915662650602No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGCGCGCCGGTA56.024096385542169No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA44.819277108433735No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA44.819277108433735No Hit
CCTGTTCGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGAA33.614457831325301No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA33.614457831325301No Hit
CCTGTTCGATACCCACACTTTCGTGCATGAGCGTCAGTTGAGCGCCGGTA22.4096385542168677No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG22.4096385542168677No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTAGGGCGCCGGTA22.4096385542168677No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA22.4096385542168677No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCATCAACGTCAGTTACTGTCCAGTA11.2048192771084338No Hit
CCTGTTCGATACCCACGCTTTCGAGCCTCAGCGTCAGTTGAACCCCGGTT11.2048192771084338No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTGCAGACCAGGA11.2048192771084338No Hit
CCTGTTCGATCCCCACGCTTTCGTGCTTCAGCGTCAGTAGGAAGCCGGCA11.2048192771084338No Hit
CCTGTTCGCTACCCACGCTTTCGTGCCTCAGCGTCAGTAGGGCGCCAGTA11.2048192771084338No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAGTGTCAGTTGCAGGCCAGTA11.2048192771084338No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAACGTCAGTCACAGTCCCGTA11.2048192771084338No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATGGTCCAGTA11.2048192771084338No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA11.2048192771084338No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGTA11.2048192771084338No Hit
CCTGTTCGATACCCACACTTTCGTGCATGAGCGTCAGTAGGGCGCCGGTA11.2048192771084338No Hit
CCTGTTTGAACCACGCACTTTCGTGGCTCAGCGTCAGTAGGGCGTCCCTA11.2048192771084338No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTGAGCGTCAGTTGTAGTTGGGTA11.2048192771084338No Hit
CCTGTTCGATAAACACGCTTTCGTGCCTGAGCGTCAGATAGGAGCCGGTA11.2048192771084338No Hit
CCTGTTTGATACCCGCACTTTCGTGCATGAGCGTCAGTTGGGCGCCGGTA11.2048192771084338No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTGAGCGTCAGTTGCGCGCCGGTA11.2048192771084338No Hit
CCTGTTCGATCCCCACGCTTTCGTGCTTCAGCGTCAGTAGGCAGCCGGCA11.2048192771084338No Hit
CATGTTCGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGCCGCCGGAC11.2048192771084338No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAGCGTCAGTTGCAGGCCAGTA11.2048192771084338No Hit
CCTGTTCGATACCCACGCTTTCGGGCCTCAGCGTCAGTAGGGCTCCGGAA11.2048192771084338No Hit
CCTGTTCGATACCCACGCTTTCGTGCATCAGCGTCAGTTGCACGCCGGTA11.2048192771084338No Hit
CCTGTTTGATCCCCACACTTTCGTGCATCAGCGTCCGTTGGGCGCCGGTC11.2048192771084338No Hit
CCTGTTGGATACCCACGCGTTCGTACCTCAGCGACAGTAACGGGACGGTA11.2048192771084338No Hit
CCTGTTTGCTACCCACACTCTCGAGCATCAGCGTCAGTTGCAGTTTGGTA11.2048192771084338No Hit
CCTGTTTGATACCCACGCTTTCGAGCTTCAGCGTCAGTTACAGACCAGAG11.2048192771084338No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTTGCGCGCCGGTA11.2048192771084338No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG11.2048192771084338No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAGTCGTCCAGTA11.2048192771084338No Hit
CCTATTAAATGCCAGCAAAACAGTTTCAAAACAACCAATTTAGAGCATTC11.2048192771084338No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAACGTCAGTAGTCCGCCGGCA11.2048192771084338No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA11.2048192771084338No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTTGCGCGCCGGAA11.2048192771084338No Hit
CCTGTTCGATACCCACACTTTCGTGCTTCAGCGTCAGTTGGGCGCCGGTA11.2048192771084338No Hit
CCTGTTCGATCCCCACGCTTTCTTGCTTCAGCGTCAGTAGGAAGCCGGCA11.2048192771084338No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTC11.2048192771084338No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTATCGTCCAGTA11.2048192771084338No Hit
CCTGTTCGCTCCCCACACTTTCGTGCATAAGCGTCAGTTGAGCGTCGGTA11.2048192771084338No Hit
CCTGTTCGATACCCACACTTTCGAGCATGAGCGTCAGTTACACGCCAGTA11.2048192771084338No Hit
CCTGTTCGATCCCCACGCTTTCGAGCCTCAGCGTCAGTAGCAAGCCAGCA11.2048192771084338No Hit
CCTGTTTGATACCCACGCTTTCGTGCCTCAACGTCAGTCGTGCTCTGGTA11.2048192771084338No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTGAGCGTCAGTAGGGCGCCGGAA11.2048192771084338No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGACAGGAGCCGGTA11.2048192771084338No Hit
CCTGTTTGATACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG11.2048192771084338No Hit
CCTGTTCGATACCCACGCTTTCGAGCATCAGTGTCAGTCGCGCTCCGGTA11.2048192771084338No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTAACCTTCATTCGCGGTCCAGCT11.2048192771084338No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA11.2048192771084338No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGGCCAGAA11.2048192771084338No Hit
CCTGTTTGATCCCCACACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA11.2048192771084338No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACACTCCAGTA11.2048192771084338No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTGAGCGTCAGTTGAGCTTTGGTA11.2048192771084338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)