FastQCFastQC Report
Fri 16 Jan 2026
M1D149_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameM1D149_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length250-251
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3137.34939759036144No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA1821.686746987951807No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCA33.614457831325301No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCA22.4096385542168677No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA22.4096385542168677No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA22.4096385542168677No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA22.4096385542168677No Hit
TACGGAGGAGGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGCA11.2048192771084338No Hit
TACGGAGGAGGCAAGCGTTATCCGGATTTATTGGGCTTAAAGGGAACGCA11.2048192771084338No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA11.2048192771084338No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGCGTAAAGGGTGCGTA11.2048192771084338No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCACTGGGCGTAAAGGGAGCGCA11.2048192771084338No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA11.2048192771084338No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA11.2048192771084338No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA11.2048192771084338No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTTTAAAGGGAGCGCA11.2048192771084338No Hit
TACGGAGGATTCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA11.2048192771084338No Hit
TACGGAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAGCGCA11.2048192771084338No Hit
TACGTAGGGGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA11.2048192771084338No Hit
TACGGAGGAGGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGATGCGTA11.2048192771084338No Hit
TACGTAGGGGGCGAGCGTTATCCGGAATTACTGGGTGTAAGGGAGGGGAG11.2048192771084338No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA11.2048192771084338No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA11.2048192771084338No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA11.2048192771084338No Hit
TACGGAGGATTCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA11.2048192771084338No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCGCGCA11.2048192771084338No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGCGCA11.2048192771084338No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA11.2048192771084338No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGAGCGTA11.2048192771084338No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA11.2048192771084338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)