FastQCFastQC Report
Fri 16 Jan 2026
F7D145_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameF7D145_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5975
Sequences flagged as poor quality0
Sequence length247-251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCACACTTTCGTGCATGAGCGTCAGTTGAGCGCCGGTA63710.661087866108787No Hit
CCTGTTCGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGAA4888.167364016736402No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA3916.543933054393306No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGCGCGCCGGTA3575.974895397489539No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA3385.656903765690377No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA3375.640167364016737No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG2664.451882845188285No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA2143.5815899581589954No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGGGCGCCGGTA1923.2133891213389125No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1652.761506276150628No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA1592.6610878661087867No Hit
CCTGTTCGATCCCCACGCTTTCGTGCTTCAGCGTCAGTAGGAAGCCGGCA1422.3765690376569037No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGGGCGCCGGTA1151.9246861924686192No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG1051.7573221757322177No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCA811.3556485355648535No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTCGGGCGCCGGTA761.2719665271966527No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTGCAGACCAGGA701.1715481171548117No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG681.1380753138075315No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGTCGTAGTTTGGTA651.0878661087866108No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA631.0543933054393306No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTCTTGGCCCAGAG540.9037656903765691No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCTGGCGCCGGTA420.7029288702928871No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGACAGGAGCCGGTA360.602510460251046No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA350.5857740585774058No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGATAGGAGCCGGTA340.5690376569037657No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA310.5188284518828452No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAC310.5188284518828452No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTG310.5188284518828452No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA290.48535564853556484No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA280.46861924686192474No Hit
CCTGTTTGCTCCCCACGCTTTCGGGCCTCAACGTCAGTCGCAGTCCAGCA270.45188284518828453No Hit
CCTGTTTGCTCCCCACGCTCTCGAGCCTCAACGTCAGTCACGGTCCAGCA270.45188284518828453No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTTCAGTCCAGCA260.4351464435146443No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTTCTGTCCAGCA230.38493723849372385No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCACCGTCCAGCA230.38493723849372385No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA220.36820083682008364No Hit
CCCGTTCGCTACCCACGCTTTCGCGCCTCAGCGTCAGGTACAGTCCAGAA220.36820083682008364No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACTGTCCAGTA210.35146443514644354No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGCA200.33472803347280333No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA200.33472803347280333No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTATCGTCCAGTA180.301255230125523No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTC180.301255230125523No Hit
CCTGTTTGCTACCCACGCTTTCGTGCTTCAGCGTCAGTTAAAGCCCAGCA170.28451882845188287No Hit
CCTGTTCGCTCCCCACGCTTTCGGGCCTCAACGTCAGTTGCCGTCCAGTA170.28451882845188287No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA170.28451882845188287No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGAA170.28451882845188287No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTTACAGTCCAGTA150.2510460251046025No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA150.2510460251046025No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA140.23430962343096237No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA130.21757322175732216No Hit
CCTGTTCGATACCCACACTTTCGTGCATGAGCGTCAGTTGCGCGCCGGTA120.20083682008368203No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACGGTCCAGCA120.20083682008368203No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA110.18410041841004182No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACGGTCCAGTA110.18410041841004182No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGACCGTCCAGTA100.16736401673640167No Hit
CCTGTTCGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA90.1506276150627615No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGCA80.13389121338912136No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTTGGCCCAGCA80.13389121338912136No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGCA80.13389121338912136No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGAA70.11715481171548119No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGAA70.11715481171548119No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTTGCAGTCCAGTT70.11715481171548119No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA70.11715481171548119No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCGCGGTCCAGCA70.11715481171548119No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCGCCGTCCAGCA60.10041841004184102No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA60.10041841004184102No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA60.10041841004184102No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGTC60.10041841004184102No Hit
CCTGTTTGCTCCCCACGCCTTCGGGCCTCAACGTCAGTCACAGTCCAGCA60.10041841004184102No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)