FastQCFastQC Report
Fri 16 Jan 2026
F6D165_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameF6D165_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5673
Sequences flagged as poor quality0
Sequence length248-251
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG492686.83236382866208No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA721.269169751454257No Hit
CCTGTTCGCTCCCCATGCTTTCGCACCCCAGCGTCGGTAGGGACCCAGAG430.7579763793407368No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATTAGTCCAGGT310.546448087431694No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG270.47593865679534636No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAA210.370174510840825No Hit
CCTGTTTGATACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG200.35254715318173807No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGCG200.35254715318173807No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGGCCAGAG140.24678300722721663No Hit
CCCATTTGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA140.24678300722721663No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGTG130.22915564956812973No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG110.19390093424995594No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGCCCAGAG90.15864621893178213No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGTCCAGAG80.14101886127269522No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACCGACCAGAG80.14101886127269522No Hit
CCTGTTTGCTCCCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG70.12339150361360832No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACAAGAG60.10576414595452141No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)