FastQCFastQC Report
Fri 16 Jan 2026
F6D165_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameF6D165_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5673
Sequences flagged as poor quality0
Sequence length250-251
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCA510690.00528820729772No Hit
GACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTA941.656971619954169No Hit
GACGGGGGGGGCAAGTGTTCTTCGGAATGACTGGGCGTAAAGGGCACGTA440.7756037369998238No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCA360.6345848757271285No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA350.6169575180680416No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA310.546448087431694No Hit
TACGTAGGAGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCA220.3878018684999119No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA190.33491979552265116No Hit
TACGGAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCA170.29966508020447735No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA130.22915564956812973No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA120.21152829190904282No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA70.12339150361360832No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA60.10576414595452141No Hit
NACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCA60.10576414595452141No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)