FastQCFastQC Report
Fri 16 Jan 2026
F5D165_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameF5D165_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2236
Sequences flagged as poor quality0
Sequence length248-251
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG190084.97316636851521No Hit
CCTGTTCGCTCCCCATGCTTTCGCACCCCAGCGTCGGTAGGGACCCAGAG462.0572450805008944No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA321.4311270125223614No Hit
CCCATTTGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA180.8050089445438283No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG140.6261180679785331No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATTAGTCCAGGT100.4472271914132379No Hit
CCTGTTTGATACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG60.26833631484794274No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG50.22361359570661896No Hit
CCCGTTCGCTCCCCATGCTTTCGCACTCCAGCGTCGGTAGGGACCCAGAG40.17889087656529518No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAA40.17889087656529518No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACGGACCAGAG40.17889087656529518No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCGGCGTCAGTTACAGACCAGAG40.17889087656529518No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGCG40.17889087656529518No Hit
ACTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG40.17889087656529518No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGACCAGAA30.13416815742397137No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGCGCGCCGGTA30.13416815742397137No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCGGTTACAGACCAGAG30.13416815742397137No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGCCCAGAG30.13416815742397137No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)