FastQCFastQC Report
Fri 16 Jan 2026
F5D165_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameF5D165_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2236
Sequences flagged as poor quality0
Sequence length248-251
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCA196988.05903398926654No Hit
GACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTA542.415026833631485No Hit
GACGGGGGGGGCAAGTGTTCTTCGGAATGACTGGGCGTAAAGGGCACGTA492.191413237924866No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA150.6708407871198568No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA120.5366726296958855No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA100.4472271914132379No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCA70.31305903398926654No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA60.26833631484794274No Hit
TACGTAGGGTCCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA50.22361359570661896No Hit
TACGTAGGAGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCA50.22361359570661896No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA40.17889087656529518No Hit
GACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCA30.13416815742397137No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCA30.13416815742397137No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)