FastQCFastQC Report
Fri 16 Jan 2026
F5D145_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameF5D145_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320
Sequences flagged as poor quality0
Sequence length249-251
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA4413.750000000000002No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGCGCGCCGGTA3611.25No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTAGGGCGCCGGTA299.0625No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGGGCGCCGGTA247.5No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA185.625No Hit
CCTGTTCGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGAA185.625No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA154.6875No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA144.375No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG134.0625No Hit
CCTGTTCGATACCCACACTTTCGTGCATGAGCGTCAGTTGAGCGCCGGTA113.4375000000000004No Hit
CCTGTTCGATCCCCACGCTTTCGTGCTTCAGCGTCAGTAGGAAGCCGGCA103.125No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTCGGGCGCCGGTA82.5No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGTCGTAGTTTGGTA61.875No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA30.9375No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGACAGGAGCCGGTA30.9375No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA20.625No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCTGGCGCCGGTA20.625No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGGGCGCCGGTA20.625No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA20.625No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG10.3125No Hit
CCTGTTCGATACCCACATTTTCGTGCATGAGCGTCAGTTGAGCGCCGGTA10.3125No Hit
CCTGTTCGATCCCCACGCTTTCGTGCTTCAGCGTCAGTTTGGCGCCGGTA10.3125No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGCGCTCCGGTA10.3125No Hit
CCTGTTCGATACCCACGCTTTCGTGCATCAGCGTCAGTAGAGCGCCGGTA10.3125No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGCGCGCTGGTA10.3125No Hit
CCTGTTTGATACCCGCACTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA10.3125No Hit
CCTGTTCGATGCCCACGCTTTCGTGCTTCAGCGTCAGTAGGGCGCCGGTA10.3125No Hit
CCTGTTTGCTCCCCACACTTTCGAGCATCAGCGTCAGTTGCGCGCCAGTA10.3125No Hit
CCTGTTCGATACCCACACTTTCGTGCATGAGCGTCAGTTGAGCGCCGGAA10.3125No Hit
ACTGCGTGATTCCCGCACTTTCGCGCCCCAGCGTCAAAAAGGCGCCGGTT10.3125No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA10.3125No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTAGGGCTCCGTTA10.3125No Hit
CCTGTTCGATACCCACGCTTTCGAGCCTCAGCGTCAGTAGGGGGCCGGTA10.3125No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTCGCGCGCCGGTA10.3125No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAATTACCCCGTAGTG10.3125No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGAA10.3125No Hit
CCTGTTTGATACACACACTTTCGAGCATCAGTGTCAGTTGCATTCCAGTG10.3125No Hit
GCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGGGCGCCGGTA10.3125No Hit
CCTGCTCGATACCCACGCTTTCGCGCCTCAGCGTCAGTCGGAAGCCGGTA10.3125No Hit
CCGGTTCGATACCCACACTTGCGTGCATGAGCGTCAGTTGAGCGCCGGTA10.3125No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTGAGCGTCAGTTGCGCGCCGGTA10.3125No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCGCCGTCCAGCA10.3125No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGGGCGCCGGAA10.3125No Hit
CCTGTTTGATGCCCTTGCTTTCGAGCCTCAACGTCCGTTACTGTCCAGCA10.3125No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT10.3125No Hit
CCTGTTCGATACCCACACTTTCGTGCATCAGCGTCAGTTGCACGCCGGTA10.3125No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTCTGGCGCCGGTA10.3125No Hit
ACTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTCGGGCGCAGGTA10.3125No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACGGTCCAGTA10.3125No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG10.3125No Hit
ATTGTTCGATACCCACACTTCCGAGCATCAGTGTCAGGTTTAGTACAGTC10.3125No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTAGCAGGCCAGAA10.3125No Hit
CCTGTTCGATACCCCCGCTTTCGTGCTTCAGCGTCAGTAGGGCTCCGGTA10.3125No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTCACAAGCCGGTC10.3125No Hit
CCCGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA10.3125No Hit
CCTGTTCGATACTCACGCTTTCGTGCTTCATCGTCAGGAGGGCCCCGGTA10.3125No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCACCGTCCAGCA10.3125No Hit
CCAATTCGCTCCCCACGCTTTCGTCCCTGAGCGTCAGTGATGGCCCAGCA10.3125No Hit
ACTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTCGGGCGCCGGTA10.3125No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACGGTCCAGCA10.3125No Hit
ACTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA10.3125No Hit
CCTGTTCGATACCCACACTTTCGTGCATGAGCTTCAGTTGGGCGCCAGTA10.3125No Hit
CCTGTTTGATACCCACACTTTCGTGCATGAGCGTCAGTTGAGCGCCGGCA10.3125No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCA10.3125No Hit
ACTGTTTGATCCCCGAAATTTCGTGCATGAGCGTCAGTTGCGCCCCGGTG10.3125No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGGGTCAGTTGCAGTCCAGTG10.3125No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTATCGTCCAGTA10.3125No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG10.3125No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTCGGCCGCCGGTA10.3125No Hit
CCTGTTTGATCCACGCACTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA10.3125No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTTCTGTCCAGCA10.3125No Hit
CCTGTTCGATACCCACACTTTCGTGCATCAGCGTCAGTTGAGCGCCGGTA10.3125No Hit
ACTGTTCGATACCCACGCTTTCGTGACTCAGCGTCAGTAGGGCGCCGGTA10.3125No Hit
CATGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA10.3125No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGATAGGCGCCGGTA10.3125No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGATAGGAGCCGGTA10.3125No Hit
CCTGTTCGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGAGCGCCGGAA10.3125No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA10.3125No Hit
CCTGTTTGATACCCACGCTTTCGAGCCTCAACGTCAGTTACAGGCCAGTA10.3125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)