FastQCFastQC Report
Fri 16 Jan 2026
F4D15_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameF4D15_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8335
Sequences flagged as poor quality0
Sequence length248-251
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA326039.112177564487105No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA189222.699460107978403No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA7328.782243551289742No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA4835.794841031793641No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCGCGCA2823.3833233353329337No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1692.027594481103779No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTA1611.931613677264547No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1261.5116976604679064No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTA580.695860827834433No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA570.6838632273545291No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCA480.5758848230353929No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA470.5638872225554888No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA460.5518896220755849No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA420.5038992201559688No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTAGGCGTAAAGCGAGTGCA380.4559088182363527No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA350.4199160167966407No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA330.3959208158368326No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCACTGGGCGTAAAGGGAGCGCA300.3599280143971206No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA200.23995200959808036No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGCGCA190.22795440911817635No Hit
TACGGAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA180.21595680863827235No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA180.21595680863827235No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA160.19196160767846432No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA160.19196160767846432No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTACGTA150.1799640071985603No Hit
TACGTAGGGAGCAAGCGTTATCCGGAATTATTGGGTGTAAAGGGTGCGTA140.16796640671865626No Hit
TACGTAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA130.15596880623875226No Hit
TACGTAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA120.14397120575884823No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA110.1319736052789442No Hit
TACGGAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCGCGCA110.1319736052789442No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCA100.11997600479904018No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA100.11997600479904018No Hit
TACGTAGGGAGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCGCGTA100.11997600479904018No Hit
TACGGAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA100.11997600479904018No Hit
TACGGAGGATGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA100.11997600479904018No Hit
TACGTAGGATCCGAGCATTATCCGGAGTGACTGGGTGTAAAGAGTTGCGT90.10797840431913618No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA90.10797840431913618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)