FastQCFastQC Report
Fri 16 Jan 2026
F4D0_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameF4D0_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5237
Sequences flagged as poor quality0
Sequence length248-251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA4628.821844567500477No Hit
CCTGTTCGATACCCACACTTTCGTGCATGAGCGTCAGTTGAGCGCCGGTA4418.420851632614092No Hit
CCTGTTCGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGAA4258.11533320603399No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACGGTCCAGTA2835.4038571701355735No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA2755.251097956845522No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGCGCGCCGGTA2725.193813251861753No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA2324.430017185411495No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1693.227038380752339No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGCAGTCCAGCA1212.3104831010120295No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG1172.234103494367004No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGTA1102.100439182738209No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGGGCGCCGGTA991.8903952644643882No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGCA941.7949207561581058No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTAGGGCGCCGGTA881.6803513461905673No Hit
CCTGTTCGATCCCCACGCTTTCGTGCTTCAGCGTCAGTAGGAAGCCGGCA851.6230666412067978No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCA711.3557380179492076No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA651.241168607981669No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGTCGTAGTTTGGTA591.1265991980141303No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCAGTCCAGTA551.0502195913691046No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTGCAGACCAGGA480.9165552797403094No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTCGGGCGCCGGTA440.8401756730952836No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTCGGGCGCCGGTA420.8019858697727706No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTATCGTCCAGTA420.8019858697727706No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAGTCGTCCAGTA380.7256062631277449No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCTGGCGCCGGTA380.7256062631277449No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTTCTGTCCAGCA360.687416459805232No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA360.687416459805232No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGGGCGCCGGTA350.6683215581439755No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTCTTGGCCCAGAG330.6301317548214627No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCGCGGTCCAGCA330.6301317548214627No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGATCGTCCAGCA300.5728470498376934No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTC300.5728470498376934No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCACCGTCCAGCA270.515562344853924No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAGAGCCCAGCA270.515562344853924No Hit
CCTGTTTGCTCCCCACGCTCTCGAGCCTCAACGTCAGTCACGGTCCAGCA250.47737254153141107No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG250.47737254153141107No Hit
CCCGTTTGCTCCCTACGCTTTCGTGCCTTAGTGTCAGAACCGTCCCAGTA250.47737254153141107No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA240.4582776398701547No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA240.4582776398701547No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGCA240.4582776398701547No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA240.4582776398701547No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACGGTCCAGTA230.4391827382088982No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA210.4009929348863853No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG210.4009929348863853No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA190.36280313156387245No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGCA180.343708229902616No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGTCGTCCAGCA170.3246133282413596No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA160.30551842658010314No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTTACAGTCCAGTA140.2673286232575902No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTTGCAGTCCAGTT140.2673286232575902No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGACCGTCCAGTA130.24823372159633378No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT120.22913881993507734No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACTGTCCAGTA120.22913881993507734No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA120.22913881993507734No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTCCAGAA120.22913881993507734No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCACCGTCCAGTA110.2100439182738209No Hit
CCTGTTTGCTACCCACGCTTTCGTGCTTCAGCGTCAGTTAAAGCCCAGCA90.171854114951308No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAATGTCAGTATCGACCCAGCA90.171854114951308No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA80.15275921329005157No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGGTCGTCCAGCA80.15275921329005157No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTTCAGTCCAGCA70.1336643116287951No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCGCCGTCCAGCA70.1336643116287951No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGTC70.1336643116287951No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGTCGTCCAGTA70.1336643116287951No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG70.1336643116287951No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACGGTCCAGCA70.1336643116287951No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTG70.1336643116287951No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGCA60.11456940996753867No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTCACTGTCCAGTA60.11456940996753867No Hit
CCTGTTCGATACCCACGCTTTCGTGCATGAGCGTCAGTTGAGCGCCGGTA60.11456940996753867No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTATCGTCCAGTC60.11456940996753867No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTACACCGTCCAGC60.11456940996753867No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG60.11456940996753867No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATGGTCCAGTA60.11456940996753867No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCGTCGTCCAGCA60.11456940996753867No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAGTAGGGCGCCGGAA60.11456940996753867No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGCCGTCCAGTA60.11456940996753867No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)