FastQCFastQC Report
Fri 16 Jan 2026
F3D13_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameF3D13_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17277
Sequences flagged as poor quality0
Sequence length248-251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA531230.746078601609078No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA522730.25409503964809No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCGCGCA12887.454997974185333No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA12767.385541471320252No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCA8194.740406320541761No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA5543.206575215604561No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA4412.5525264802917174No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2641.5280430630317765No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1650.9550269143948601No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1330.7698095734213116No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTA1170.6772009029345373No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA1120.6482606934074203No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA530.30676622098743994No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA350.20258146668981886No Hit
GACGGGGGGGGCAAGTGTTCTTCGGAATGACTGGGCGTAAAGGGCACGTA340.19679342478439543No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA330.19100538287897206No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTAGGCGTAAAGCGAGTGCA320.18521734097354867No Hit
TACGGAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCGCGCA300.17364125716270187No Hit
TACGGAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCA300.17364125716270187No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATCACTGGGCGTAAAGGGCGCGTA290.16785321525727848No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA250.14470104763558486No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGCGTAAAGGGAACGCA220.1273369219193147No Hit
TACGTAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA220.1273369219193147No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA220.1273369219193147No Hit
TACGTAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA210.1215488800138913No Hit
TACGGAGGATGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGCGCGCA200.1157608381084679No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA190.10997279620304451No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)